Objective To investigate the cluster of Candida auris (C. auris) healthcare-associated infection (HAI)in a pediatric intensive care unit (PICU), clarify its transmission mechanism, identify prevention and control loopholes, and develop precise prevention and control strategies, so as to provide evidence-based support for the prevention and control of C. auris infection in children. Methods Epidemiological investigation were conducted on two children from whom C. auris were detected. Surveillance on environmental hygiene and active screening on high-risk children were carried out. Potential failure modes were identified by healthcare failure mode and effect analysis (HFMEA), prevention and control strategies were developed and the effectiveness of implementation were assessed. Results C. auris were detected from one healthcare worker (HCW)’s hand and one high-risk child’s groin swab. HFMEA identified 8 high-risk failure modes, including inadequate awareness of HCWs, missed admi-ssion screening, and non-standard hand hygiene. After implementing prevention and control strategies, key indicators improved significantly, including the qualified rate of environmental hygiene surveillance, implementation rate of isolation measures, compliance rate of hand hygiene, and pathogen detection rate (all P<0.05), with no new cases reported for 5 months. Conclusion Hand contact among HCWs is the key link in the transmission of C. auris in PICU. HFMEA can effectively identify risks and control transmission. The closed-loop management model (surveillance-risk assessment-precise intervention-effectiveness verification) established in this study can provide reference for the prevention and control of C. auris infection in children.