Objective To detect carbapenemase types in strains isolated from patients with carbapenem-resistant Enterobacterales (CRE) infection, analyze bacterial resistance, clinical characteristics of infected patients, and related factors affecting patients’ prognosis. Methods Non-repetitive CRE strains isolated from adult inpatients in a secondary first-class general hospital from 2023 to 2024 were collected prospectively. Carbapenemase types were detected, patients’ clinical data were investigated, and factors affecting the clinical treatment outcome of patients with carbapenemase-procucing Enterobacterales (CPE) infection were analyzed with univariate and multivariate regression models. Results Clinical data of 151 CRE infected patients were collected, the detection of carbapenem-resistant Klebsiella pneumoniae (CRKP) took the highest proportion (n=134, 88.7%). All of the 151 CRE strains contained carbapenemase, including 111 strains(73.5%)containing only class A serine carbapenemase (all KPC type), 32 strains (21.2%)containing only class B metallo-β-lactamase (MBL), and 8 strains (5.3%)containing both KPC and MBL(double-carbapenemase-producing, DCP). KPC represented the main form in CRKP (82.1%). Both carbapenem-resistant Escherichia coli (CREC) and carbapenem-resistant Enterobacter cloacae (CRECL) produced MBL. The resistance rates of 151 CPE strains to ampicillin/sulbactam, piperacillin/tazobactam, cefoperazone/sulbactam, and ticarcillin/clavulanic acid were all over 90%. The resistance rates to ceftazidime/avibactam in only-MBL-producing strains and DCP strains were higher than those in only-KPC-producing strains (both P<0.05). The resistance rates of only-KPC-producing and DCP strains to aminoglycosides, doxycycline, and compound sulfamethoxazole were higher than those of only-MBL-producing strains (all P<0.05). All three types of CPE strains had good sensitivity to polymyxin B, with a resistance rate of 0-4.2%. The resistance rates of KPC- and MBL-producing strains to tigecycline were low (0-4.2%), while the resistance rate of DCP strains to tigecycline was 100%. 27.2% (n=41) of patients died within 30 days after infection. Multivariate regression analysis showed that healthcare-associated infection (HAI) (OR=12.88, 95% CI: 4.15-39.96), indwelling gastric tube (OR=10.51, 95% CI: 2.19-50.45), and high abdominal blood glucose level during infection (OR=1.24, 95% CI: 1.08-1.41) were all independent risk factors for death within 30 days after infection in patients with CPE infection, while high serum albumin level during infection (OR=0.80, 95% CI: 0.70-0.90) was an independent protective factor. Conclusion The prevalence of CPE in secondary general hospitals is high, and antimicrobial resistance is severe, especially in the cases of KPC-producing and DCP strains showing wider spectrum of antimicrobial resistance. Attention should be paid to CRE screening and enzyme type monitoring to prevent HAI. High-risk populations should also be paid attention to improve clinical prognosis.