Objective To investigate the usage status of trunk protective isolation products and wearing feeling of isolation gowns made of different materials in tertiary dental hospitals, and provide evidence for optimizing the usage of isolation gowns. Methods A questionnaire survey was conducted to collect data on the usage of isolation gowns and white coats (including type, replacement frequency, cleaning costs, etc.) from 16 tertiary stomatological specialty hospitals. The wearing feeling on three types of isolation gowns (disposable, self-made reusable, and commercial reusable) of healthcare workers (HCWs) in 4 outpatient departments of a hospital was evaluated. Results All institutions used both white coats and isolation gowns, with 81.25% using both one-piece and two-piece white coats, and 75.00% of the institutions used both disposable and reusable isolation gowns. During splashing procedures, 87.50% of the institutions adopted the practice of wearing scrubs underneath isolation gowns. Isolation gowns were mostly replaced daily(75.00%), white coats 1-2 times weekly(43.75%), and scrubs varied in replacement frequency. 87.50% of the institutions outsourced reusable textiles for cleaning. For reusable isolation gowns, the maximum monthly usage reached 21 346 pieces, the median monthly usage per dental unit and per HCW were close to the numbers of white coats. For white coats, the median monthly usage was the highest (2 537 pieces), and the median cleaning cost per piece was also the highest (￥3.50). The highest purchasing cost was from reusable isolation gowns (￥85.00) and the lowest was from disposable ones (￥12.50). Disposable gowns received poor subjective evaluation for breathability, stretchability, and odor during use; self-made reusable gowns received poor evaluation in weight and effective blocking of blood and body fluid; commercial reusable gowns received poor evaluation for being "too short in length". Key improvement suggestions on three isolation gowns focused on breathability, weight, and protective coverage. Conclusion Isolation gowns are recommended during dental splash procedures. The overall cost of purchasing as well as monthly cleaning and disinfection is relatively high. The design of isolation gowns should balance protective performance and comfort based on clinical needs in dental practice.