Objective To develop evaluation indicators and a scale for assessing the implementation of the infection control link nurse (ICLN) program based on the RE-AIM framework, and to explore strategies for improving implementation by evaluating the implementation of the ICLN program in a tertiary grade A hospital. Methods Based on the RE-AIM framework, evaluation indicators and a scale for evaluating implementation strategies of the ICLN program were developed. The evaluation scale was used to conduct a cross-sectional survey of all ICLNs in a tertiary grade A hospital where the ICLN program had been implemented for 10 years. Results The evaluation indicators and scale covered 10 items across five dimensions: Reach, Effectiveness, Adoption, Implementation, and Maintenance. The survey results showed that: in terms of Reach, the proportion of nursing units with an ICLN was 100%, while the standardized ICLN establishment rate was 44.14%. Regarding Effectiveness, among the core work contents of the ICLN program, except for revising policies and procedures, the scores for the other three items were above 4 points. Regarding Adoption, among the adoption scores of different personnel groups for ICLN training, physicians had the lowest score, at 4.32±0.73 points. Regarding Implementation, self-audit and feedback had the highest implementation rates (both 100%), while the implementation rate for reviewing policies and procedures was the lowest (37.24%). Regarding Maintenance, the mean score for willingness to serve as an ICLN was 4.40±0.82 points. Conclusion The RE-AIM-based ICLN evaluation indicators and scale can be used to comprehensively evaluate the implementation of the ICLN program and provide a basis for further research on ICLN implementation strategies.